What Techniques are Used for Single Cell Analysis?
Several advanced techniques are employed for single cell analysis:
- Single Cell RNA Sequencing (scRNA-seq): This method sequences the RNA of individual cells, providing a comprehensive view of gene expression. - Flow Cytometry and Fluorescence-Activated Cell Sorting (FACS): These techniques are used to sort and analyze cells based on specific markers. - Mass Cytometry (CyTOF): Combines flow cytometry and mass spectrometry to measure multiple parameters per cell. - Single Cell Western Blotting: Allows for the analysis of protein expression at the single cell level.