Several techniques are employed to isolate cellular components, including:
Differential Centrifugation: This method uses varying centrifugal forces to separate cellular components based on their size and density. Density Gradient Centrifugation: This technique involves layering a cell lysate on a density gradient and centrifuging it, which separates components based on their buoyant density. Ultracentrifugation: High-speed centrifugation used for separating smaller cellular components such as ribosomes and various types of vesicles. Flow Cytometry: A method that sorts cells based on their physical and chemical characteristics. Immunoprecipitation: Uses antibodies to isolate specific proteins or complexes from a mixture.
