What Methods Are Used to Study Proton Motive Force in Histology?
Several techniques are employed to study PMF in histological samples. These include:
Fluorescent dyes: Specific dyes can measure pH gradients and membrane potential, providing insights into PMF dynamics. Electron microscopy: High-resolution imaging can reveal structural details of mitochondria, correlating with functional data. Respirometry: This technique measures oxygen consumption rates, indirectly reflecting ETC activity and PMF.