The tyramide reaction, also known as tyramide signal amplification (TSA), is a powerful biochemical technique used in histology to amplify signals in immunohistochemistry (IHC) and in situ hybridization (ISH). This method enhances the detection sensitivity of target molecules such as proteins, nucleic acids, and other biomolecules. Tyramide is a small aromatic compound that, when activated by a peroxidase enzyme, forms highly reactive intermediates that covalently bind to nearby phenolic groups, effectively amplifying the signal.
