Tyramide signal amplification offers several advantages:
- Increased Sensitivity: TSA can detect low-abundance targets that are otherwise difficult to visualize with conventional methods. - Reduced Background Noise: The covalent binding of activated tyramide to target molecules results in a higher signal-to-noise ratio. - Versatility: TSA is compatible with a wide range of detection systems including fluorescence, biotin, and streptavidin-based systems. - Multiplexing: TSA allows for the simultaneous detection of multiple targets in a single sample, enabling complex studies of cellular processes.
