Several factors can influence the precision of sectioning:
Tissue Preparation: Proper fixation, dehydration, and embedding are critical for maintaining tissue integrity and consistency. Microtome Quality: A well-maintained microtome with a sharp blade is essential for cutting uniform sections. Technique: The skill and experience of the technician play a significant role in achieving precise sections. Temperature: Maintaining appropriate temperature conditions can prevent artifacts such as freezing artifacts in cryosectioning.
