To achieve precision in sectioning, consider the following steps:
Optimal Fixation: Ensure the tissue is properly fixed to preserve its structural integrity. Common fixatives include formalin and glutaraldehyde. Embedding Medium: Use an appropriate embedding medium, such as paraffin wax for routine histology or OCT compound for cryosectioning. Sectioning Technique: Adjust the microtome settings to achieve the desired thickness and use a gentle, consistent cutting motion. Blade Maintenance: Regularly change and sharpen the microtome blade to ensure clean cuts. Environmental Control: Maintain consistent temperature and humidity in the sectioning room to avoid artifacts.
