Initial Adjustment: Start with the coarse focus knob to bring the sample into general focus. This is typically done using the lowest magnification objective lens. Fine Adjustment: Once the initial focus is achieved, use the fine focus knob to sharpen the image. This step is crucial for higher magnifications. Objective Lens: Switch to higher magnification lenses as needed, readjusting the focus with the fine focus knob each time. Condenser and Diaphragm: Adjust the condenser and diaphragm settings to optimize illumination and contrast, which can improve focus quality.
