Several factors can impact the durability of histological specimens:
Fixation: Proper fixation is vital for preserving tissue morphology and preventing autolysis and putrefaction. Common fixatives include formaldehyde, alcohol, and glutaraldehyde. Embedding: The choice of embedding medium, such as paraffin wax or resin, can affect the long-term stability of the tissue samples. Staining: The durability of stains, such as hematoxylin and eosin (H&E), depends on the quality of reagents and the staining protocols used. Mounting: Proper mounting techniques and the use of appropriate mounting media can protect stained sections and enhance their longevity. Storage: Environmental conditions like temperature, humidity, and light exposure can significantly impact the preservation of histological samples.
