Several advanced technologies are employed in the process of 3D reconstruction:
- Confocal Microscopy: This technique allows for the collection of high-resolution, optically sectioned images that are ideal for 3D reconstruction. - Multiphoton Microscopy: Useful for imaging deeper into tissues, this method minimizes photodamage and photobleaching. - Serial Block-Face Scanning Electron Microscopy (SBF-SEM): Provides ultrastructural details by imaging the block face of a resin-embedded sample as it is sectioned. - Optical Coherence Tomography (OCT): Offers real-time, non-invasive imaging suitable for reconstructing the 3D architecture of tissues.
