Several factors can influence cell viability in histological samples:
- Sample Handling: Poor handling can cause physical damage to cells, reducing viability. - Fixation: While fixation is necessary to preserve tissue structure, improper fixation can lead to cell death. - Temperature: Cells are sensitive to temperature fluctuations, which can affect their viability. - Oxygen Levels: Hypoxia or inadequate oxygen supply can lead to cell death. - Nutrient Availability: Cells require adequate nutrients to maintain their viability and function.
