What are the Challenges in Assessing Cell Viability?
Despite the availability of various techniques, assessing cell viability in histological samples presents challenges:
- Artifact Formation: Fixation and staining can sometimes create artifacts that mimic cell death. - Heterogeneity: Tissue samples often contain a mix of different cell types with varying sensitivities, complicating the assessment. - Quantification: Accurately quantifying live versus dead cells in complex tissues can be difficult.
