Several methods are employed to measure cell viability, including:
- Trypan Blue Exclusion Assay: A dye exclusion method where viable cells exclude the dye, while dead cells absorb it. - MTT and XTT Assays: These colorimetric assays measure metabolic activity as an indicator of cell viability. - Flow Cytometry: Uses fluorescent dyes to distinguish between live and dead cells based on membrane integrity and metabolic activity. - Live/Dead Staining: Utilizes fluorescent dyes that can penetrate only dead cells, allowing for easy distinction under a microscope.
