What are the Applications of Collagenase Type I in Histology?
Tissue Dissociation: Collagenase Type I is used to dissociate tissues into single-cell suspensions, a critical step in preparing samples for flow cytometry, immunohistochemistry, and other analytical techniques. Cell Isolation: The enzyme aids in isolating specific cell types from tissue samples. For example, isolating hepatocytes from liver tissue or fibroblasts from skin samples. Regenerative Medicine: In regenerative medicine, collagenase Type I is used to prepare scaffolds and cell-seeded constructs for tissue repair and regeneration. Culturing Primary Cells: Primary cells often require a collagenase treatment to detach from the extracellular matrix, facilitating their use in cultures and experiments.
