Several factors can influence the viability of cell cultures:
Nutrient Availability: Adequate supply of essential nutrients and growth factors is critical for maintaining cell health and proliferation. Environmental Conditions: Factors such as temperature, pH, and oxygen levels must be carefully controlled to ensure optimal cell growth and viability. Contamination: Bacterial, fungal, or viral contamination can drastically reduce cell viability and compromise experimental results. Cell Density: Both overcrowding and under-seeding can negatively impact cell growth and viability, requiring careful monitoring and adjustment.
