Several methods are available to assess cell culture viability:
Trypan Blue Exclusion: This dye is excluded by viable cells but penetrates and stains dead cells, making it a straightforward and cost-effective method. MTT Assay: This colorimetric assay measures cellular metabolic activity as an indicator of viability, relying on the reduction of MTT by mitochondrial enzymes in living cells. Flow Cytometry: This technique uses fluorescent dyes to differentiate between live and dead cells, providing a high-throughput and precise assessment of cell viability. Lactate Dehydrogenase (LDH) Release: This assay measures the release of LDH enzyme from damaged cells, indicating cell membrane integrity and viability.
