What Challenges are Associated with Sectioning Thinness?
Achieving optimal section thinness can be challenging due to several factors: - Tissue Hardness: Hard tissues like bone require decalcification before sectioning. - Temperature: Maintaining the correct temperature is crucial, especially for cryostat sectioning. - Skill Level: Precision sectioning requires a high level of skill and experience. - Artifacts: Improper sectioning can introduce artifacts, which may obscure important details.
