What Challenges are Associated with Adherent Cell Culture?
Culturing adherent cells poses several challenges:
1. Substrate Selection: Choosing the appropriate substrate for cell attachment is critical for maintaining cell viability and function. 2. Contamination: Adherent cell cultures are susceptible to contamination by bacteria, fungi, and mycoplasma, which can compromise the quality of the research. 3. Cell Line Variability: Different cell lines may exhibit variations in attachment and growth characteristics, requiring optimization of culture conditions for each cell type. 4. Passaging: Adherent cells require regular passaging to prevent over-confluence, which can lead to changes in cell behavior and function.
