Improper Fixation: Fixation is crucial for preserving tissue morphology. Inadequate or improper fixation can lead to poor staining outcomes. Incorrect Reagent Preparation: Using expired or improperly prepared reagents can compromise staining quality. Inadequate Dehydration: Insufficient removal of water from tissue sections before staining can affect stain uptake. pH Imbalance: The pH of staining solutions must be carefully controlled. Deviations from the optimal pH range can result in suboptimal staining. Poor Technique: Inconsistent or incorrect application of staining protocols can lead to errors. Environmental Factors: Temperature and humidity variations can affect staining outcomes.
