Despite its benefits, coating in histology presents several challenges:
Uniformity: Achieving a consistent and even coating can be difficult, which may affect the quality of microscopic examination. Compatibility: Not all coating materials are compatible with all types of tissues or staining techniques. Selecting the appropriate material is essential. Processing Time: Some coating methods, especially embedding with resins, can be time-consuming, requiring careful planning and execution. Technical Expertise: Proper application of coatings requires skill and experience, as improper techniques can lead to artifacts or damage to the specimen.
