The H&E staining process involves several key steps:
Fixation: Tissue samples are fixed to preserve their structure and prevent degradation. Embedding: Fixed tissues are embedded in paraffin wax to create a solid block for sectioning. Sectioning: Thin sections of the tissue block are cut using a microtome. Staining: Sections are first stained with hematoxylin to visualize the nuclei, followed by eosin to stain the cytoplasm and extracellular components. Mounting: Stained sections are mounted on glass slides with a coverslip for microscopic examination.
