The detection of Hb Bart's typically involves a combination of histological techniques and laboratory assays. Hemoglobin electrophoresis and high-performance liquid chromatography (HPLC) are commonly used to identify and quantify abnormal hemoglobins. In a histological context, tissue samples from the liver, spleen, or bone marrow may be examined under a microscope to assess the extent of erythropoiesis and the presence of abnormal erythrocytes, which are indicative of thalassemia syndromes.
