What are the Limitations of Phenol Chloroform Extraction?
Despite its advantages, this method has some limitations:
Labor-Intensive: The process involves multiple steps and requires careful handling to avoid contamination. Toxic Reagents: Phenol and chloroform are hazardous chemicals that require proper safety precautions during use and disposal. RNA Degradation: RNA is more susceptible to degradation, and extra care is needed to avoid contamination with RNases.
