How to Ensure Successful Phenol Chloroform Extraction?
To achieve successful extraction, consider the following tips:
Use Fresh Samples: Fresh or properly preserved samples yield better results. Maintain RNase-Free Conditions: Use RNase inhibitors and work in an RNase-free environment when extracting RNA. Proper Centrifugation: Ensure proper phase separation by using appropriate centrifugation speeds and times. Careful Phase Collection: Carefully collect the aqueous phase without disturbing the interface to avoid contamination.
