What are the Limitations of Enzymatic Techniques in Histology?
While enzymatic techniques are powerful, they have certain limitations. Enzyme activity can be affected by tissue processing methods such as fixation, leading to potential loss of activity. The specificity of enzymatic reactions may also pose challenges, as some substrates can be acted upon by multiple enzymes, leading to ambiguous results. Additionally, the quantification of enzyme activity in histological sections can be complex and requires careful standardization.
