What is Immunohistochemistry and How Do Enzymes Play a Role?
Immunohistochemistry (IHC) is a technique that combines histological, immunological, and biochemical methods to identify specific antigens in tissue sections. Enzymes like horseradish peroxidase (HRP) and alkaline phosphatase are often conjugated to antibodies. These enzyme-linked antibodies bind to the target antigen, and the enzymatic reaction produces a detectable signal, usually a color change, allowing for the visualization of the antigen.
