During the sectioning of tissue blocks, the temperature of both the tissue block and the microtome blade is vital. Cold tissue blocks are easier to section because they are firmer. Cryostat microtomes, which operate at sub-zero temperatures, allow for the sectioning of frozen tissue samples, preserving enzyme activity and antigenicity. Conversely, room temperature or higher can make sectioning more challenging and may lead to tearing or distortion of the tissue.
