Several key areas in histology require standardization:
1. Tissue Preparation: This includes fixation, embedding, sectioning, and staining. Each step must be carefully controlled to avoid artifacts and ensure reproducibility. 2. Staining Techniques: The choice of stains and staining protocols needs to be consistent to enable accurate comparison of tissue morphology and structures. 3. Microscopy: Standardizing microscope settings, including magnification, illumination, and imaging techniques, is crucial for consistent observations. 4. Image Analysis: Digital imaging and quantitative analysis techniques need standardization to provide reliable data.
