What are the Common Components of Blocking Solutions?
Blocking solutions often include proteins, detergents, or a combination of both. Some commonly used blocking agents are:
- Serum: Derived from the same species as the secondary antibody to prevent cross-reactivity. - Bovine Serum Albumin (BSA): A widely used protein blocker. - Casein: A milk-derived protein. - Gelatin: Another protein source used in blocking. - Non-ionic Detergents: Such as Triton X-100 or Tween-20, which can help reduce background by blocking hydrophobic interactions.
