Preparation of a blocking solution is straightforward but must be done with care to avoid contamination. Here is a general protocol:
1. Select the Blocking Agent: Decide whether you will use serum, BSA, casein, or a detergent. 2. Dissolve the Blocking Agent: Typically, blocking agents are dissolved in a buffer such as PBS (Phosphate Buffered Saline) or TBS (Tris Buffered Saline). 3. Filter the Solution: Use a 0.22 μm filter to remove any particulate matter. 4. Store Appropriately: Store the solution at 4°C and use it within a reasonable timeframe to avoid microbial growth.
