Choosing the appropriate blocking solution depends on several factors, including the tissue type, the primary antibody, and the detection method. Here are some considerations:
- Tissue Type: Different tissues may have varying levels of non-specific binding. For example, brain tissue often requires more stringent blocking due to its lipid content. - Primary Antibody: The species and isotype of the primary antibody can influence the choice of blocking agent. For example, serum from the same species as the secondary antibody is often recommended. - Detection Method: Enzyme-based detection systems might require different blocking agents compared to fluorescence-based methods.
