A typical plasmid vector includes several key components:
An origin of replication (ori), which allows the plasmid to replicate within the host cell. A selectable marker gene, such as antibiotic resistance, to identify cells that contain the plasmid. A multiple cloning site (MCS), which contains several restriction enzyme sites for easy insertion of the gene of interest. Regulatory elements, such as promoters and enhancers, to control the expression of the inserted gene.
