What Are the Common Techniques Used in Live Cell Imaging?
Several techniques are employed in live cell imaging, each with its unique advantages:
Fluorescence Microscopy: This technique uses fluorescent dyes or proteins to label cellular components, allowing visualization of specific structures within living cells. Confocal Microscopy: A form of fluorescence microscopy that provides high-resolution images by eliminating out-of-focus light, making it ideal for thick tissue samples. Time-Lapse Microscopy: This method captures a series of images over time, enabling the study of dynamic processes such as cell movement and division. Multiphoton Microscopy: Utilizing longer wavelengths of light, this technique allows deeper tissue penetration, making it suitable for imaging live tissues in three dimensions.
