What are the Challenges in Using γH2AX as a Biomarker?
Despite its widespread use, there are challenges associated with using γH2AX as a biomarker. The interpretation of γH2AX foci can be complex due to variations in staining intensity and background noise. Additionally, the presence of γH2AX does not always correlate directly with irreversible DNA damage, as it can also mark transient breaks that are quickly repaired. Therefore, complementary assays are often required to obtain a comprehensive assessment of DNA damage.
