Staining agents are vital for highlighting different structures within tissue sections. These dyes and chemicals bind specifically to various cellular components, making them visible under a microscope. Hematoxylin and Eosin (H&E) are the most commonly used stains in histology. Hematoxylin stains cell nuclei blue, while eosin stains cytoplasm and extracellular matrix pink. Other specialized stains include Periodic Acid-Schiff (PAS) for carbohydrates and Masson's Trichrome for connective tissue.
