Resolution: Achieving high resolution is critical for detecting fine details in tissue sections. However, higher resolution often comes at the cost of longer acquisition times and larger data files. Contrast: Adequate contrast is necessary to distinguish different tissue components. Staining techniques and image processing can enhance contrast, but improper methods can lead to artifacts. Artifacts: These are distortions or errors in the image that can result from sample preparation, staining, or imaging techniques. Minimizing artifacts is essential for accurate interpretation.
