Despite its advantages, optical clearing also presents several challenges:
1. Tissue Damage: Some clearing agents can cause tissue shrinkage or damage, affecting the integrity of the sample. 2. Fluorescence Quenching: Certain agents may quench fluorescent signals, complicating imaging. 3. Compatibility: Not all clearing agents are compatible with all types of tissues or staining protocols. 4. Time-Consuming: The clearing process can be time-consuming, particularly for large or dense tissues.
