To obtain reliable results, it is important to optimize the conditions of the MTT assay:
Cell Density: Ensure that the cell density is within an optimal range to avoid over or underestimation of viability. Incubation Time: Optimize the incubation time for the conversion of MTT to formazan to ensure maximum sensitivity. Reagent Concentration: Use the appropriate concentration of MTT reagent to achieve the best results. Control Wells: Include wells with known numbers of viable and non-viable cells to serve as controls.
