What are the advantages of FLIM over traditional fluorescence microscopy?
FLIM offers several advantages over traditional fluorescence microscopy, including:
Higher specificity: FLIM can distinguish between fluorophores with similar emission spectra but different lifetimes. Environmental sensitivity: Fluorescence lifetime is sensitive to the local environment, providing information on pH, ion concentration, and molecular interactions. Reduced photobleaching: Since FLIM relies on lifetime rather than intensity, it can reduce the effects of photobleaching.
