What Are the Limitations of Apoptosis Detection Methods?
Each method has limitations: - H&E Staining: Non-specific and may not distinguish apoptosis from necrosis. - TUNEL Assay: Can generate false positives due to DNA repair processes. - Annexin V: May not distinguish between apoptosis and necrosis without additional markers. - Caspase Assays: Require confirmation with other markers to ensure specificity.
