Several factors can contribute to inadequate adhesion in histological procedures:
1. Improper Slide Preparation: The surface of the glass slide must be clean and appropriately treated to promote tissue adherence. Any contamination or improper coating can result in poor adhesion. 2. Tissue Processing Errors: Over-fixation or under-fixation of tissues can compromise their structural integrity, making it difficult for them to adhere to slides. 3. Inappropriate Adhesives: The type of adhesive used must be suitable for the specific tissue type and staining protocol. Inappropriate choices can lead to detachment during processing. 4. Environmental Factors: Humidity and temperature can affect the adhesive properties of tissues and slides. Extreme conditions can exacerbate adhesion problems.
