Tissue fixation is a crucial step in histology, as it preserves the morphology and molecular integrity of tissues. Key guidelines include: 1. Choice of Fixative: Use appropriate fixatives like formalin for routine histology, or glutaraldehyde for electron microscopy. 2. Fixation Time: Ensure adequate fixation time, generally 24-48 hours for formalin, to prevent autolysis and degradation. 3. Fixative Volume: The volume of fixative should be at least 10 times the volume of the tissue specimen. 4. Temperature: Perform fixation at room temperature to enhance penetration and fixation quality.
