Tissue processing involves dehydration, clearing, and infiltration. Key protocols include: 1. Dehydration: Use a graded series of alcohols to remove water from the tissue. 2. Clearing: Use xylene or substitutes to make the tissue transparent and ready for infiltration. 3. Infiltration: Embed tissues in paraffin wax to provide support for thin sectioning. 4. Automation: Utilize automated tissue processors to enhance consistency and efficiency.
