What are the best practices for tissue sectioning?
Sectioning involves cutting thin slices of tissue for microscopic examination. Best practices include: 1. Microtome Calibration: Ensure the microtome is well-calibrated and maintained. 2. Section Thickness: Cut sections at an appropriate thickness, typically 4-5 microns for light microscopy. 3. Blade Quality: Use sharp, clean blades to produce smooth, artifact-free sections. 4. Section Handling: Carefully transfer sections to slides to avoid wrinkles and tears.
