Several best practices can enhance the quality of sectioning:
Proper Alignment: Ensure the tissue is properly aligned in the microtome to achieve even sections. Sharp Blades: Use sharp, clean blades to avoid tearing or compressing the tissue. Consistent Thickness: Maintain consistent section thickness, usually between 3-5 micrometers for paraffin-embedded tissues. Controlled Environment: Work in a controlled environment to prevent changes in temperature and humidity that may affect sectioning quality. Regular Maintenance: Regularly maintain and calibrate the microtome to ensure optimal performance.
