Polyadenylation sites are specific regions in the mRNA where a poly(A) tail is added. This process is crucial for the stability, export, and translation of mRNA. Polyadenylation is a post-transcriptional modification that occurs in the nucleus of eukaryotic cells. The addition of a poly(A) tail to the 3' end of an mRNA molecule involves cleavage of the pre-mRNA at a specific site, followed by the addition of a sequence of adenine nucleotides.