The process of staining involves several steps to ensure the tissue is properly prepared and the stain is applied effectively:
Fixation: Preserves the tissue and prevents degradation. Common fixatives include formalin and ethanol. Embedding: Tissues are embedded in a medium, usually paraffin, to provide support during sectioning. Sectioning: Thin slices of the embedded tissues are cut using a microtome. Staining: The tissue sections are placed on slides and stained using the chosen method. Mounting: The stained tissue is covered with a coverslip for examination under a microscope.
