Validating the specificity of an antibody is a critical step in histological studies. This can be done by:
Western Blotting: To confirm that the antibody recognizes a protein of the expected size. Immunoprecipitation: To verify that the antibody can pull down the target antigen from a mixture of proteins. Immunohistochemistry and Immunocytochemistry: To ensure the antibody stains the correct cellular structures. Knockout or Knockdown Experiments: Using cells or tissues lacking the target antigen to demonstrate the absence of staining or signal.
