To achieve optimal results in immunofluorescence experiments, consider the following tips: - Antibody Selection: Choose high-quality, well-validated primary and secondary antibodies. - Blocking: Use appropriate blocking agents to minimize non-specific binding. - Controls: Include positive and negative controls to validate the specificity of the staining. - Fluorescence Filters: Use the correct filters for the fluorescent dyes used to avoid overlap and cross-talk between channels. - Mounting Media: Use mounting media that reduce photobleaching and preserve fluorescence.
