How to Optimize EDTA Buffer for Specific Applications?
Optimization of EDTA buffer for specific histological applications involves adjusting parameters such as concentration, pH, and incubation time. For decalcification, it is crucial to monitor the process regularly to avoid over-decalcification. For antigen retrieval, the temperature and duration of heating should be optimized for each specific antibody and tissue type. It is also important to validate the procedure by including appropriate positive and negative controls.
